| Rabbit Anti-HSD11B2 antibody |
| 反应物种（预测） |
Dog |
| 产品应用（已验证） |
WB,ICC,FCM |
| 推荐稀释比例 |
WB=1:200-1000,Flow Cyt=1ug/Test,ICC=1:100, |
| 研究领域 |
肿瘤,免疫学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSD11B2 Polyclonal Antibody, Unconjugated(bs-3618R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Formalin-fixed and paraffin embedded horse kidney labeled with Anti- HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded rat kidney labeled with Anti- HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-HSD11B2 (bs-3618R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
RRID：AB_10857692
产品名称：Rabbit Anti-HSD11B2 antibody
别名： Corticosteroid 11 beta dehydrogenase isozyme 2; 11 beta HSD2; 11 beta hydroxysteroid dehydrogenase type 2; 11 DH2; AME; AME1; HSD11K; HSD2; Hydroxysteroid 11 beta dehydrogenase 2; Hydroxysteroid 11 beta dehydrogenase isoenzyme 2; NAD dependent 11 beta hyd
中文名称：羟基类固醇脱氢酶11β2抗体
英文名称：Rabbit Anti-HSD11B2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human HSD11B2
抗原表位：151-250/405
SWISS：P80365
Gene ID ：3291
Human Gene ID：3291
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]
Function：Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Subunit：Interacts with ligand-free cytoplasmic NR3C2.
Subcellular Location：Microsome. Endoplasmic reticulum.
Tissue Specificity：Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
Similarity：Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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