| Rabbit Anti-CD40/TNFRSF5 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
心血管,细胞生物,免疫学,干细胞,细胞表面分子,淋巴细胞,B-淋巴细胞, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (bs-2929R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Rat) Lysate at 40 ug
Primary: Anti-CD40/TNFRSF5 (bs-2929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 50 kD
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Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-CD40(bs-2929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 52 kD
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Sample:
Jurkat(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-CD40(bs-2929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 52 kD
RRID：AB_11070685
产品名称：Rabbit Anti-CD40/TNFRSF5 antibody
别名： Tumor necrosis factor receptor superfamily, member 5; TNFRSF5; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40 antigen (TNF receptor superfamily member 5); CD40 antig
中文名称：肿瘤坏死因子受体超家族成员5抗体
英文名称：Rabbit Anti-CD40/TNFRSF5 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CD40/TNFRSF5.
抗原表位：201-277/277
抗原细胞定位：Cytoplasmic
SWISS：P25942
Gene ID ：958
Human Gene ID：958
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul2008].
Subcellular Location：Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.
Tissue Specificity：B-cells and in primary carcinomas.
Similarity：Contains 4 TNFR-Cys repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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