Rabbit Anti-Histone H1.4 (Acetyl K53) antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse |
产品应用(已验证) |
IHC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500, |
研究领域 |
肿瘤,发育生物学,信号转导,细胞凋亡,转录调节因子,表观遗传学, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Molt-4 cells were fixed with 4% PFA for 10min at room temperature ,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H1.4 (Acetyl K53) Antibody(bs-0931R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10857291
产品名称:Rabbit Anti-Histone H1.4 (Acetyl K53) antibody
别名: Histone H1.4(Ac-Lys53); Histone H1b (Ac-Lys53); Acetyl-Histone H1.4 (Lys53); Acetyl-Histone H1.4 (K53); HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4; H1 histone family member 4; H1E; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; H
中文名称:乙酰化组蛋白H1b抗体
英文名称:Rabbit Anti-Histone H1.4 (Acetyl K53) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised acetylpeptide derived from human Histone H1b around the acetylation site of Lys53
抗原表位:AS(Ac-K)ER
SWISS:P10412
Gene ID :3008
Human Gene ID:3008
Histone H1b are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Function:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Subcellular Location:Nucleus. Chromosome.
Post-translational modifications:Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Similarity:Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.