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Rabbit Anti-GDF15 antibody
多克隆  |   SKU:bs-3818R

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货号:bs-3351R
¥1280
订购号:bs-3818R
¥1098.00-2900.00
货期:现货
Rabbit Anti-GDF15 antibody
产品应用(已验证)

WB,IHC

产品应用(可尝试)

IF,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,

研究领域

心血管,细胞生物,信号转导,细胞周期蛋白,细胞分化,

标签

Array

  • Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (bs-3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
  • Sample:
    K562 Cell Lysate at 30 ug
    A431 Cell Lysate at 30 ug
    Primary: Anti-GDF15 (bs-3818R) at 1:300 dilution;
    Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
    Predicted band size:34 kD
    Observed band size:30 kD
  • Sample:
    Lane 1: Urinary bladder (Mouse) Lysate at 40 ug
    Lane 2: Large intestine (Mouse) Lysate at 40 ug
    Lane 3: Urinary bladder (Rat) Lysate at 40 ug
    Lane 4: Large intestine (Rat) Lysate at 40 ug
    Lane 5: HT1080 (Human) cell Lysate at 40 ug
    Lane 6: HepG2 (Human) cell Lysate at 40 ug
    Lane 7: K562 (Human) cell Lysate at 40 ug
    Lane 8: A549 (Human) cell Lysate at 40 ug
    Primary: Anti-GDF15 (bs-3818R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 35 kD
    Observed band size: 35 kD

产品信息

RRID:AB_10857699
产品名称:Rabbit Anti-GDF15 antibody
别名: GDF 15; GDF-15; GDF15_HUMAN; Growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; Mic-1; MIC1; NAG 1; NAG-1; NAG1; NRG 1; NRG-1; NRG1; NSAID (nonsteroidal anti inflammatory drug) acti
中文名称:生长分化因子15/巨嗜细胞抑制因子1抗体
英文名称:Rabbit Anti-GDF15 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human GDF-15
抗原表位:221-308/308
SWISS:Q99988
Gene ID :9518
Human Gene ID:9518

产品介绍

Growth differentiation factor 15 (GDF15) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. It has been implicated in a variety of functions directly related to tumorigenicity including antiproliferative and pro-apoptotic effects. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF15 is an important downstream mediator of DNA damage signaling and a transcriptional target of p53.
Subcellular Location:Secreted.
Tissue Specificity:Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Similarity:Belongs to the TGF-beta family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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