| Rabbit Anti-Phospho-Cyclin B1 (Ser147) antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,细胞周期蛋白 |
| 标签 |
Array |
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Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Cyclin B1(Ser147) Polyclonal Antibody, Unconjugated(bs-3122R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control (blue line): A549 (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-Cyclin B1 (Ser147) antibody(bs-3122R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-Cyclin B1 (Ser147) antibody (bs-3122R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10857939
产品名称：Rabbit Anti-Phospho-Cyclin B1 (Ser147) antibody
别名： Cyclin B1 (phospho S147); Cyclin B1 (phospho-Ser147); p-Cyclin B1 (S147); p-Cyclin B1 (Ser147); p-Cyclin B1 (phospho S147); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
中文名称：磷酸化周期素B1抗体
英文名称：Rabbit Anti-Phospho-Cyclin B1 (Ser147) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147
抗原表位：AF(p-S)DV
SWISS：P14635
Gene ID ：891
Human Gene ID：891
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Function：Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subunit：Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active
Subcellular Location：Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications：Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosph
Similarity：Belongs to the cyclin family. Cyclin AB subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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