Rabbit Anti-phospho-SIRT6 (Ser338) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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磷酸化抗体

Rabbit Anti-phospho-SIRT6 (Ser338) antibody

多克隆 | SKU：bs-5634R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-5634R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-phospho-SIRT6 (Ser338) antibody
产品应用（已验证）	IHC,ICC
产品应用（可尝试）	WB,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100,
研究领域	肿瘤,心血管,免疫学,染色质和核信号,信号转导,细胞凋亡,新陈代谢,表观遗传学,
标签	Array

Paraformaldehyde-fixed, paraffin embedded (human ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT6(Ser338)) Polyclonal Antibody, Unconjugated (bs-5634R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-phospho-SIRT6(Ser338) antibody (bs-5634R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-APC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：AB_11117859
产品名称：Rabbit Anti-phospho-SIRT6 (Ser338) antibody
别名： SIRT6(phospho S338); NAD-dependent protein deacylase sirtuin-6; SIR2L6; EC:2.3.1.-; NAD-dependent protein deacetylase sirtuin-6; Protein mono-ADP-ribosyltransferase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIR6_HUMAN;
中文名称：磷酸化沉默调节相关蛋白6抗体
英文名称：Rabbit Anti-phospho-SIRT6 (Ser338) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthesised phosphopeptide derived from human SIRT6 around the phosphorylation site of Ser338
抗原表位：PT(p-S)PA
SWISS：Q8N6T7
Gene ID ：51548
Human Gene ID：51548

产品介绍

NAD-dependent protein deacetylase. Has deacetylase activity towards 'Lys-9' and 'Lys-56' of histone H3. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates 'Lys-9' of histone H3 at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. Regulates the production of TNF protein.
Function：NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and
Subunit：Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8.
Subcellular Location：Nucleus, nucleoplasm.
Similarity：Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.