Rabbit Anti-API5 antibody |
反应物种(预测) |
Human,Dog,Pig,Cow,Horse,GuineaPig |
产品应用(已验证) |
WB,IHC |
推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500, |
研究领域 |
肿瘤,免疫学,细胞凋亡 |
标签 |
Array |
-
Sample:
Heart (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-API5 (bs-1252R) at 1:300
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000 dilution
Predicted band size : 57 kD
Observed band size :57 kD
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10886464
产品名称:Rabbit Anti-API5 antibody
别名: apoptosis inhibitor 5; apoptosis inhibitory protein 5; API5; AAC-11; AAC11; API5L1; AAC 11; Antiapoptosis clone 11 protein; API5 like 1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; Migration inducing prote
中文名称:凋亡抑制因子5抗体
英文名称:Rabbit Anti-API5 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human API5
抗原表位:351-460/504
SWISS:Q9BZZ5
Gene ID :8539
Human Gene ID:8539
Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. API5 expression is often upregulated in tumor cells, particularly in metastatic cells. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription. Alternate Names: AAC 11; AAC11; Antiapoptosis clone 11 protein; API 5; API5; API5 like 1; API5L1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL.
Function:Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its de
Subunit:Monomer. Interacts with FGF2 and ACIN1.
Subcellular Location:Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic.
Isoform 3: Cytoplasm.
Tissue Specificity:Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma
Post-translational modifications:Acetylation at Lys-251 impairs antiapoptotic function.
Similarity:Belongs to the API5 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.