| Rabbit Anti-NCF1 antibody |
| 反应物种（预测） |
Rat,Dog |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/test, |
| 研究领域 |
肿瘤,细胞生物,免疫学,新陈代谢, |
| 标签 |
Array |
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U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCF1 Antibody(bs-3886R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-NCF1 (bs-3886R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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Sample:
Bone (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-NCF1 (bs-3886R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
RRID：AB_10855771
产品名称：Rabbit Anti-NCF1 antibody
别名： 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1;
中文名称：嗜中性粒细胞胞浆因子1抗体
英文名称：Rabbit Anti-NCF1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human NCF1
抗原表位：151-250/390
SWISS：P14598
Gene ID ：653361
Human Gene ID：653361
NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).
Function：NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Subunit：Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG.
Subcellular Location：Cytoplasm.
Post-translational modifications：Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.
DISEASE：Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1) [MIM:233700]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacte
Similarity：Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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