| Rabbit Anti-Phospho-YB1 (Ser102) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转录调节因子 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-YB1 (Ser102)) Polyclonal Antibody, Unconjugated (bs-3477R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-YB1 (Ser102)) Polyclonal Antibody, Unconjugated (bs-3477R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-YB1 (Ser102)) Polyclonal Antibody, Unconjugated (bs-3477R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-YB1(Ser102) (bs-3477R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
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Sample:
Hela(human) Cell Lysate at30 ug
Primary: Anti-Phospho-YB1(Ser102) (bs-3477R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
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phosphopeptide
non phosphopeptide
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Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-YB1(Ser102)) Polyclonal Antibody, Unconjugated (bs-3477R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10856150
产品名称：Rabbit Anti-Phospho-YB1 (Ser102) antibody
别名： Y box-binding protein1; nuclease sensitive element binding protein 1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1; MGC104858; MGC110976; MGC117250; YBX1; CCAAT binding transcription factor I subunit A; DNA binding protein B; major histocomp
中文名称：磷酸化核酸酶敏感元件结合蛋白1抗体
英文名称：Rabbit Anti-Phospho-YB1 (Ser102) antibody
中文别名：DNA结合蛋白B; y-盒结合蛋白1;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human YB1 around the phosphorylation site of Ser102
抗原表位：LR(p-S)VG
SWISS：P67809
Gene ID ：4904
Human Gene ID：4904
The Y-box binding protein 1(YB1)is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumour aggressiveness and belongs to the cold-shock domain family.
Function：Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA an
Subunit：Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified i
Subcellular Location：Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance
Post-translational modifications：Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.
In the absence of phosphorylation the protein is retained in the cytoplasm.br
Similarity：Contains 1 CSD (cold-shock) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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