Rabbit Anti-Phospho-LKB1 (Thr189) antibody |
反应物种(预测) |
Mouse,Rat,Pig,Cow |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
肿瘤,免疫学,神经生物学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
标签 |
Array |
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Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-LKB1(Thr189) (bs-3250R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: THP-1 (Human) Cell Lysate at 30 ug
Lane 3: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-LKB1 (Thr189) (bs-3250R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/48 kD
Observed band size: 48/46 kD
RRID:AB_10855758
产品名称:Rabbit Anti-Phospho-LKB1 (Thr189) antibody
别名: STK11(phospho T189); LKB1(phospho Thr189); LKB1 (phospho T189); p-LKB1 (phospho T189); p-LKB1(phospho Thr189); p-LKB1(T189); LKB1; PJS; Renal carcinoma antigen NY-REN-19; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine pro
中文名称:磷酸化丝氨酸/苏氨酸蛋白激酶抗体
英文名称:Rabbit Anti-Phospho-LKB1 (Thr189) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human LKB1 around the phosphorylation site of Thr189
抗原表位:G(p-T)LK
SWISS:Q15831
Gene ID :6794
Human Gene ID:6794
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008].
Function:Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts
Subunit:Catalytic component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Found in a ternary compl
Subcellular Location:Nucleus. Cytoplasm. Membrane. Mitochondrion. Note=A small fraction localizes at membranes. Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to mitochondrion during apoptosi
Tissue Specificity:Ubiquitously expressed. Strongest expression in testis and fetal liver.
Post-translational modifications:Phosphorylated by ATM at Thr-363 following ionizing radiations (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylat
DISEASE:Defects in STK11 are a cause of Peutz-Jeghers syndrome
(PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melan
Similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.