| Rabbit Anti-APOA1 antibody |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
心血管,神经生物学,信号转导,脂蛋白,新陈代谢, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIBP) Polyclonal Antibody, Unconjugated (bs-4573R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Apolipoprotein A 1) Polyclonal Antibody, Unconjugated (bs-4573R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse intestines); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Apolipoprotein A 1) Polyclonal Antibody, Unconjugated (bs-4573R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
Serum(Rat) Lysate at 40 ug
Plasma(Rat) Lysate at 40 ug
Primary: Anti-Apolipoprotein A 1 (bs-4573R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
RRID：RRID
产品名称：Rabbit Anti-APOA1 antibody
别名： Apo-AI;
ApoA I;
ApoA-I;
APOA1_HUMAN;
Apolipoprotein A-I(1-242);
Apolipoprotein A1;
Apolipoprotein A 1;
Apolipoprotein AI;
Apolipoprotein A I;
Brp14;
Ltw1;
Lvtw1;
Sep1;
Sep2.
中文名称：载脂蛋白a1抗体
英文名称：Rabbit Anti-APOA1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Apolipoprotein A 1
抗原表位：45-150/267
SWISS：P02647
Gene ID ：335
Human Gene ID：335
Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Function：Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activat
Subunit：Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1.
Subcellular Location：Secreted.
Tissue Specificity：Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
Post-translational modifications：Palmitoylated.
Phosphorylation sites are present in the extracelllular medium.
DISEASE：Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in h
Similarity：Belongs to the apolipoprotein A1/A4/E family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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