Rabbit Anti-phospho-Cyclin D3 (Thr283) antibody |
反应物种(预测) |
Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
ICC,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1ug/Test,ICC=1:100, |
研究领域 |
肿瘤,细胞生物,染色质和核信号,细胞周期蛋白,表观遗传学, |
标签 |
Array |
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A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Cyclin D3 (Thr283)) polyclonal Antibody, Unconjugated (bs-12155R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID:RRID
产品名称:Rabbit Anti-phospho-Cyclin D3 (Thr283) antibody
别名: Cyclin D3 (phospho T283); Cyclin D3 (phospho Thr283); p-Cyclin D3 (T283); p-Cyclin D3 (Thr283); p-Cyclin D3 (phospho T283); CCND 3; Ccnd3; CCND3_HUMAN; CyclinD3; D3 type cyclin; G1 S specific cyclin D3; G1/S specific cyclin D3; G1/S-specific cyclin-D3.
中文名称:磷酸化周期素D3抗体
英文名称:Rabbit Anti-phospho-Cyclin D3 (Thr283) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human Cyclin D3 around the phosphorylation site of Thr283
抗原表位:TS(p-T)PT
SWISS:P30281
Gene ID :896
Human Gene ID:896
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008].
Function:Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of t
Subunit:Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CD
Subcellular Location:Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
Post-translational modifications:Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
Similarity:Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.