Rabbit Anti-NTR3 antibody |
反应物种(预测) |
Human,Dog,Pig,Cow,Horse,GuineaPig |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
心血管,细胞生物,免疫学,神经生物学,细胞凋亡 |
标签 |
Array |
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Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-NTR3 (bs-6329R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
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Sample: Brain (Rat) Lysate at 40 ug
Primary: Anti-NTR3 (bs-6329R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
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Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NTR3 Polyclonal Antibody, Unconjugated(bs-6329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NTR3) Polyclonal Antibody, Unconjugated (bs-6329R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:RRID
产品名称:Rabbit Anti-NTR3 antibody
别名: 100 kDa NT receptor; Glycoprotein 95; Gp 95; Gp95; Neurotensin receptor 3; NT 3; NT3; NTR 3; NTR3; SORT 1; SORT_HUMAN; SORT1 (gene name); SORT1; Sortilin 1; Sortilin; Sortilin1.
中文名称:神经降压素受体3/糖蛋白95抗体
英文名称:Rabbit Anti-NTR3 antibody
抗体来源: Rabbit
克隆类型:多克隆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Sortilin/NTR3/Gp95
抗原表位:731-831/831
SWISS:Q99523
Gene ID :6272
Human Gene ID:6272
Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
Tissue specificity: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
Function:Functions as a sorting receptor in the Golgi compartmentand as a clearance receptor on the cell surface. Required forprotein transport from the Golgi apparatus to the lysosomes by apathway that is independent of the mannose-6-phosphate receptor(M6PR). Als
Subunit:Interacts with LPL and SLC2A4 (By similarity). Interactswith the cytosolic adapter proteins GGA1 and GGA2. Interacts withnumerous ligands including the receptor-associated proteinLRPAP1/RAP, GM2A, NTS and PSAP. Forms a complex with NGFR whichbinds specifi
Subcellular Location:Membrane; Single-pass type I membraneprotein. Endoplasmic reticulum membrane; Single-pass type Imembrane protein (Potential). Endosome membrane; Single-pass type Imembrane protein (Potential). Golgi apparatus, Golgi stackmembrane; Single-pass type I membr
Tissue Specificity:Expressed at high levels in brain, spinalcord, heart, skeletal muscle, thyroid, placenta and testis.Expressed at lower levels in lymphoid organs, kidney, colon andliver.
Post-translational modifications:The N-terminal propeptide is cleaved by furin and possiblyother homologous proteases.
DISEASE:Note=A common polymorphism located in a non-coding regionbetween CELSR2 and PSRC1 alters a CEBP transcription factor bindingsite and is responsible for changes in hepatic expression of SORT1.Altered SORT1 expression in liver affects low density lipoprotei
Similarity:Belongs to the VPS10-related sortilin family. SORT1subfamily.
Contains 9 BNR repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.