| Rabbit Anti-phospho-IRS1 (Ser323) antibody |
| 反应物种（预测） |
Rat,Rabbit |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
WB,IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,糖尿病,细胞膜蛋白, |
| 标签 |
Array |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser323) antibody (bs-12709R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Sample:Ovary (Mouse) Lysate at 40 ug
Primary: Anti-phospho-IRS1 (Ser323)(bs-12709R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132kD
Observed band size: 132kD
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser323) antibody (bs-12709R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser323)) Polyclonal Antibody, Unconjugated (bs-12709R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-phospho-IRS1 (Ser323) antibody
别名： IRS1 (phospho S323); p-IRS1 (phospho S323); HIRS 1;
IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
中文名称：磷酸化胰岛素受体底物1抗体
英文名称：Rabbit Anti-phospho-IRS1 (Ser323) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser323
抗原表位：PG(p-S)FR
SWISS：P35568
Gene ID ：3667
Human Gene ID：3667
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Function：May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosp
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vasc
Post-translational modifications：Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.
DISEASE：The gene represented in this entry may be involved in disease pathogenesis.
Disease description:A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body
Similarity：Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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