Mouse Anti-beta I Tubulin (Loading Control) antibody |
产品应用(已验证) |
WB,IHC,ICC,FCM |
产品应用(可尝试) |
IF |
推荐稀释比例 |
WB=1:5000-20000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
细胞生物,免疫学,信号转导,细胞骨架, |
标签 |
Array |
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Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Fetal brain (Mouse) Lysate at 40 ug
Lane 5: Heart (Mouse) Lysate at 40 ug
Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 7: Spleen (Rat) Lysate at 40 ug
Lane 8: Spleen (Mouse) Lysate at 40 ug
Lane 9: Ovary (Rat) Lysate at 40 ug
Primary: Anti-beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0296G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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Sample:
293T(Human) Cell Lysate at 30 ug
Cerebellum (Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample:
K562 Cell (Human) Lysate at 40 ug
Jurkat Cell (Human) Lysate at 40 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M 5F7) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
RRID:RRID
产品名称:Mouse Anti-beta I Tubulin (Loading Control) antibody
别名: 2810484G07Rik;
Beta tubulin 1, class VI;
Class VI beta tubulin;
dJ543J19.4;
M(beta)1;
TBB1_HUMAN;
TUBB1;
Tubulin beta 1 class VI;
Tubulin beta-1 chain;
Tubulin, beta 1;
tubulin, beta1;
中文名称:微管蛋白β1 tubulin(内参)单克隆抗体
英文名称:Mouse Anti-beta I Tubulin (Loading Control) antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG2a
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human beta I Tubulin
SWISS:Q9H4B7
Gene ID :81027
Human Gene ID:81027
This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]
Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Subunit:Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtub
Subcellular Location:Cytoplasm, cytoskeleton
Tissue Specificity:Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.
Post-translational modifications:Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes
DISEASE:Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1). The disease is caused by mutations affecting the gene represented in this entry. A congenital blood disorder characterized by increased platelet size and decreased number of circulating
Similarity:Belongs to the tubulin family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.