| Rabbit Anti-STAT6 antibody |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,ICC,IF |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:20-200,IHC-F=1:20-200,Flow Cyt=2ug/Test,IF=1:20-200,ICC=1:20-200, |
| 研究领域 |
肿瘤,发育生物学,信号转导,细胞凋亡,转录调节因子,表观遗传学, |
| 标签 |
Array |
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Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- STAT6 (bsm-52239R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kD
Observed band size: 100 kD
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Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-Phospho-Akt1(Ser473) antibody (bsm-52130R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-Phospho-Akt1(Ser473) antibody (bsm-52130R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-STAT6 antibody
别名： signal transducers and activators of transduction 6; D12S1644; IL 4 STAT; IL4 STAT; Interleukin 4 Induced; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced; Signal Transducer And
中文名称：信号转导和转录激活因子6重组兔单克隆抗体
英文名称：Rabbit Anti-STAT6 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human STAT6 protein, around 700-800aa
SWISS：P42226
Gene ID ：6778
Human Gene ID：6778
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X (L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
Function：Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Subunit：Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 via its C-terminal LXXLL motif.
Subcellular Location：Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Post-translational modifications：Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
Similarity：Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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