| Rabbit Anti-AGR2 antibody |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IP |
| 推荐稀释比例 |
WB=1:1000-5000,IP=1:20-100,IHC-P=1:50-200,IHC-F=1:50-200,ICC=1:50-200, |
| 研究领域 |
肿瘤,细胞生物,免疫学,转录调节因子 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Primary:
Anti-AGR2 (bsm-52594R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 15 kD
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGR2) Monoclonal Antibody, Unconjugated (bsm-52594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-AGR2 antibody
别名： HAG2; AG2 protein; hAG2; Secreted cement gland protein XAG2 homolog; AG 2; AG 2 protein; AG-2; AG2; AG2 protein; AGR 2; AGR2; AGR2_HUMAN; Anterior gradient 2 homolog (Xenopus laevis); anterior gradient 2 homolog; Anterior gradient protein 2 homolog; GOB 4
中文名称：前梯度同源蛋白2重组兔单克隆抗体
英文名称：Rabbit Anti-AGR2 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞浆,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human AGR2
SWISS：O95994
Gene ID ：10551
Human Gene ID：10551
AGR2 is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and ?dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.
Function：Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Subunit：Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked.
Subcellular Location：Secreted. Endoplasmic reticulum.
Tissue Specificity：Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid
Similarity：Belongs to the AGR family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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