Rabbit Anti-Phospho-JNK1/2/3(T183+T183+T221) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

订购号:bsm-52462R

￥1580.00-2680.00

50ul

100ul

货期：现货

Rabbit Anti-Phospho-JNK1/2/3(T183+T183+T221) antibody
产品应用（已验证）	WB,IHC,ICC,FCM
产品应用（可尝试）	IP
推荐稀释比例	WB=1:1000-2000,IP=1:20-50,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=1:100,ICC=1:50,
研究领域	肿瘤,信号转导,转录调节因子
标签	Array

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with ( Phospho-JNK1/2/3(T183+T183+T221)) monoclonal Antibody, Unconjugated (bsm-52462R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK1/2/3(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human uterus tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK1/2/3(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK1/2/3(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: NIH/3T3 cell lysate, treated with Anisomycin
Lane 2: NIH/3T3 cell lysate, untreated
Primary: Anti-Phospho-JNK1/2/3(T183+T183+T221) (bsm-52462R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 42 kD
Observed band size: 54/46 kD
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with ( Phospho-JNK1/2/3(T183+T183+T221)) monoclonal Antibody, Unconjugated (bsm-52462R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (bsm-52462R)
Dilution: 1:100;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-JNK1/2/3(T183+T183+T221)) monoclonal Antibody, Unconjugated (bsm-52462R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK123(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK123(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Uterine Corpus Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK123(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-JNK123(T183+T183+T221)) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with ( Phospho-JNK1/2/3(T183+T183+T221)) monoclonal Antibody, Unconjugated (bsm-52462R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

产品信息

RRID：RRID
产品名称：Rabbit Anti-Phospho-JNK1/2/3(T183+T183+T221) antibody
别名： JNK1 + JNK2 + JNK3 (phospho T183 + T183 + T221); JNK1 (phospho T183); p-JNK1 (phospho T183); MAPK8 (phospho T183); JNK1 + JNK2 (phospho Thr183 + Thr183); JNK1 + 2 (phospho Thr183+Thr183); p-JNK; c Jun N terminal kinase 1; C-JUN kinase 1; EC 2.7.11.24; JAK
中文名称：磷酸化氨基末端激酶1/2/3重组兔单克隆抗体
英文名称：Rabbit Anti-Phospho-JNK1/2/3(T183+T183+T221) antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性状：Lyophilized or Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated Synthesised phosphopeptide derived from human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221
抗原表位：MM(p-T)PY
SWISS：P45983
Gene ID ：5599
Human Gene ID：5599

产品介绍

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
Function：Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress
Subcellular Location：Cytoplasm. Nucleus.
Post-translational modifications：Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
Similarity：Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.