| Rabbit Anti-CYP26A1 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-1000,Elisa=1:5000-10000,IHC-P=1:50-200,IHC-F=1:50-200, |
| 研究领域 |
肿瘤,细胞生物,神经生物学,信号转导,新陈代谢, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP26A1) Monoclonal Antibody, Unconjugated (bsm-54341R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP26A1) Monoclonal Antibody, Unconjugated (bsm-54341R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-CYP26A1 (bsm-54341R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
RRID：RRID
产品名称：Rabbit Anti-CYP26A1 antibody
别名： CP26; CP26A_HUMAN; CYP26; CYP26A 1; cyp26a1; Cytochrome P450 26A1; Cytochrome P450 family 26 subfamily A polypeptide 1; Cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, family 26, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIA,
中文名称：细胞色素cP450 CYP26A1重组兔单克隆抗体
英文名称：Rabbit Anti-CYP26A1 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human CYP26A1
抗原表位：200-400/497
SWISS：O43174
Gene ID ：1592
Human Gene ID：1592
The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP26A1 is a major retinoic acid catabolic enzyme. CYP26A1 plays an important role in protecting tailbud tissues from inappropriate exposure to retinoic acid. CYP26A1 transcription is epigenetically regulated by nuclear retinoic acid receptor ∫2. Mutations in the gene encoding for CYP26A1 are associated with caudal agenesis and spina bifida, imperforate anus, agenesis of the caudal portions of the digestive and urogenital tracts, and malformed lumbosacral skeletal elements. CYP26A1 is upregulated in adenomatous polyposis coli mouse adenomas, human FAP adenomas, human sporadic colon carcinomas, and in the intestine of adenomatous polyposis coli (apcmcr) mutant zebrafish embryos.
Function：Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, in
Tissue Specificity：Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.
Similarity：Belongs to the cytochrome P450 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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