Mouse Anti-Histone H3 (tri methyl K4) antibody |
反应物种(预测) |
Mouse,Rat |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:200-500, |
研究领域 |
染色质和核信号,表观遗传学, |
标签 |
Array |
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Sample:
Lane 1: Human SH-SY5Y Cell lysates
Lane 2: Human 293T Cell lysates
Primary: Anti- Histone H3 (tri methyl K4) (bsm-33855M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
RRID:RRID
产品名称:Mouse Anti-Histone H3 (tri methyl K4) antibody
别名: Histone H3 (tri methyl Lys4) ; H3K4me3;
H3 histone family member E pseudogene;
H3 histone family, member A;
H3/A;
H31_HUMAN;
H3F3;
H3FA;
Hist1h3a;
HIST1H3B;
HIST1H3C;
HIST1H3D;
HIST1H3E;
HIST1H3F;
HIST1H3G;
HIST1H3H;
HIST1H3I;
HIST1H3J;
H
中文名称:甲基化组蛋白H3(tri methyl K4)单克隆抗体
英文名称:Mouse Anti-Histone H3 (tri methyl K4) antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG1
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K4
抗原表位:RT(Tri Methyl-K)QT
SWISS:P68431
Gene ID :8350
Human Gene ID:8350
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic
Subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular Location:Nucleus; Chromosome
Tissue Specificity:Expressed in testicular cells.Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
Post-translational modifications:Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by E
Similarity:Belongs to the histone H3 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.