Rabbit Anti-CD34 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

首页 > 产品 > 一抗 > 产品信息

Rabbit Anti-CD34 antibody

多克隆 | SKU：bs-0646R

说明书

联系我们

打印此页面

货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
相关产品
相关文献

订购号:bs-0646R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-CD34 antibody
反应物种（预测）	Rat,Dog,Pig,Cow,Rabbit
产品应用（已验证）	WB,IHC,ICC,IF,FCM
产品应用（可尝试）	ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:200-800,
研究领域	肿瘤,心血管,免疫学,发育生物学,神经生物学,干细胞,细胞表面分子,糖蛋白,细胞类型标志物(Neuron marker, Soma marker, Myeloid Cells),血管内皮细胞,内皮细胞,
标签	Array

Sample:
Huvec(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti-CD34 (bs-0646R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Blank control: HUVEC.
Primary Antibody (green line): Rabbit CD34 antibody (bs-0646R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD34 Polyclonal Antibody, Unconjugated(bs-0646R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human gastric adenocarcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD34 Polyclonal Antibody, Unconjugated(bs-0646R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37°C.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD34 Polyclonal Antibody, Unconjugated(bs-0646R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Raji(blue).
Primary Antibody:Rabbit Anti- CD34 antibody(bs-0646R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-0646R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-0646R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Sample:
MCF-7(Human) Cell Lysate at 40 ug
Primary: Anti-CD34 (bs-0646R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 115 kD

产品信息

RRID：AB_10857521
产品名称：Rabbit Anti-CD34 antibody
别名： CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.
中文名称：CD34抗体
英文名称：Rabbit Anti-CD34 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human CD34
抗原表位：301-385/385
抗原细胞定位：Extracellular
SWISS：P28906
Gene ID ：947
Human Gene ID：947

产品介绍

The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Function：Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Post-translational modifications：Highly glycosylated.
Phosphorylated on serine residues by PKC.
Similarity：Belongs to the CD34 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.