Rabbit Anti-CD24 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-CD24 antibody

多克隆 | SKU：bs-0528R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0528R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-CD24 antibody
反应物种（预测）	Mouse
产品应用（已验证）	WB,FCM
产品应用（可尝试）	ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /test,
研究领域	肿瘤,细胞生物,免疫学,神经生物学,干细胞,细胞粘附分子,细胞表面分子,糖蛋白,T-淋巴细胞,B-淋巴细胞,骨髓细胞,
标签	Array

Blank control: A549 (blue).
Primary Antibody:Rabbit Anti-CD24 antibody(bs-0528R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0528R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD24 Polyclonal Antibody, Unconjugated(bs-0528R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Colon carcinoma (Human) lysate at 30ug;
Heart(Rat) lysates, 30ug;
Primary: Anti-CD24 (bs-0528R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 8kD
Observed band size : 35kD

产品信息

RRID：AB_10856894
产品名称：Rabbit Anti-CD24 antibody
别名： Signal transducer CD24; CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Small cell lung carcinoma cluster 4 antigen; Ly-52; CD24_HUMAN.
中文名称：CD24抗体
英文名称：Rabbit Anti-CD24 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human CD24 Propeptide
抗原表位：60-80/80
SWISS：P25063
Gene ID ：100133941
Human Gene ID：100133941

产品介绍

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. [provided by RefSeq, Jul 2008].
Function：Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-
Subcellular Location：Cell membrane.
Tissue Specificity：B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Post-translational modifications：Extensively O-glycosylated.
DISEASE：Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes an
Similarity：Belongs to the CD24 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.