| Rabbit Anti-DLL4 antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,神经生物学,细胞周期蛋白,细胞膜受体 |
| 标签 |
Array |
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Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Delta 4 Polyclonal Antibody, Unconjugated(bs-6044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane 1: Human K562 cell Lysates
Lane 2: Human THP-1 cell Lysates
Lane 3: Human 293T cell Lysates
Primary: Anti-DLL4 (bs-6044R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75kDa
Observed band size: 75kDa
RRID：AB_11051322
产品名称：Rabbit Anti-DLL4 antibody
别名： DeltaD; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); Drosophila Delta homolog 4; delta-like 4 homolog; delta-like 4 protein; Aei; After eight; dld; DLL4_HUMAN; Delta-like protein 4; Drosophila Delta homolog 4; Delta 4 precursor; d
中文名称：δ样蛋白4抗体
英文名称：Rabbit Anti-DLL4 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human DLL4
抗原表位：551-650/685
SWISS：Q9NR61
Gene ID ：54567
Human Gene ID：54567
The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged-2 and Delta. While blocking the differentiation of progenitor cells into the B-cell lineage, Delta promotes the emergence of a population of cells with T cell/NK-cell characteristics. The protein is a membrane protein expressed in heart, pancreas, brain and muscle during gastrulation and early organogenesis and in adult heart and lung. Delta-4 is a membrane protein that activates Notch-1 and Notch-4. It is expressed in a wide range of adult and fetal tissues, especially in vascular endothelium.
Function：Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
Subunit：Binds to Notch-1 and Notch-4 (By similarity).
Subcellular Location：Membrane; Single-pass type I membrane protein (Probable).
Tissue Specificity：Expressed in vascular endothelium.
Post-translational modifications：Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
Similarity：Contains 1 DSL domain.
Contains 8 EGF-like domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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